Structure of a molluscan hemocyanin didecamer (HtH1 from Haliotis tuberculata ) at 12 Å resolution by cryoelectron microscopy
Identifieur interne : 003692 ( Main/Exploration ); précédent : 003691; suivant : 003693Structure of a molluscan hemocyanin didecamer (HtH1 from Haliotis tuberculata ) at 12 Å resolution by cryoelectron microscopy
Auteurs : Ulrich Meissner [Allemagne] ; Prakash Dube [Allemagne] ; J. Robin Harris [Allemagne] ; Holger Stark [Allemagne] ; Jürgen Markl [Allemagne]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 2000.
English descriptors
- KwdEn :
- Teeft :
- Angular reconstitution, Biol, Breemen, Bruggen, Central tier, Characteristic views, Class averages, Collar components, Cryoelectron, Cryoelectron microscopy, Decamer, Didecamer, Didecamers, Dimer, Electron density, Electron microscopy, Fourier shell correlation function, Fscf, Functional units, Gastropod, Gebauer, Haliotis, Haliotis tuberculata, Helix pomatia, Hemocyanin, Hemocyanin molecule, Hemocyanins, Holde, Holde miller, Horizontal sections, Hth1, Hth1 decamer, Hth1 didecamer, Hth1 subunit dimer, Hth2, Image processing, Interactive extraction, Keller, Keyhole, Keyhole limpet hemocyanin, Klh1, Klh2, Klug, Lamy, Lieb, Limpet, Markl, Mellema, Mellema klug, Micrographs, Micron, Molecule, Molluscan, Molluscan hemocyanin decamer, Molluscan hemocyanins, Morphological unit, Morphological units, Oblique, Octopus, Octopus hemocyanin, Orlova, Outer surface, Pomatia, Power spectrum, Projection image, Quaternary, Quaternary structure, Sohngen, Stereo pairs, Structural feature, Structural features, Subunit, Subunit dimer, Thin layer, Third tier, Threshold curve, Tier, Tuberculata, Unstained molecules, Upper decamer, White arrows.
Abstract
Abstract: A 12 Å resolution three-dimensional density map of the Haliotis tuberculata hemocyanin type 1 (HtH1) didecamer has been obtained by cryoelectron microscopy of unstained molecules and angular reconstitution. The dyad symmetry of the 8 MDa D5 HtH1 didecamer, formed by the pairing of two asymmetric 4 MDa ring-like C5 decamers, is emphasised. The major and minor surface helical grooves of the didecamer are well defined, in agreement with earlier data on molluscan hemocyanins. The location of the obliquely orientated repeating unit, a subunit dimer, within the decamer has been defined. Following interactive extraction of this dimer, several new structural features of the dimer and of the subunit have now emerged with improved detail. The subunit dimer possesses pseudo 2-fold symmetry, resulting from the steric arrangement of the wall domains/functional units (FUs-abcdef) of the two subunits. The arc and collar FUs (g and h) depart from this inherent 2-fold symmetry and are thereby responsible for the asymmetry of the C5 decamer, with the internalised collar/arc complex at one edge of the decamer. The FU heterodimers forming the wall morphological units have a hollow centre, and thus create a series of repeating channels that extend within the wall through all three tiers of the decamer. The connections between the wall and the arc are defined with improved clarity, and evidence is provided to indicate that the arc and collar FU pairs have a homodimeric composition (gg and hh, respectively). Two possibilities for the subunit path within the subunit dimer are presented, which correlate with the available structural, immunolabelling and protease cleavage data from HtH1 and other molluscan hemocyanins.
Url:
DOI: 10.1006/jmbi.2000.3631
Affiliations:
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Robin Harris</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Zoology University of Mainz D-55099, Mainz</wicri:regionArea><wicri:noRegion>55099, Mainz</wicri:noRegion><wicri:noRegion>Mainz</wicri:noRegion></affiliation></author><author><name sortKey="Stark, Holger" sort="Stark, Holger" uniqKey="Stark H" first="Holger" last="Stark">Holger Stark</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Molecular Biology and Tumour Research Emil-Mannkopff-Strasse 2 D-35037</wicri:regionArea></affiliation></author><author><name sortKey="Markl, Jurgen" sort="Markl, Jurgen" uniqKey="Markl J" first="Jürgen" last="Markl">Jürgen Markl</name><affiliation></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Zoology University of Mainz D-55099, Mainz</wicri:regionArea><wicri:noRegion>55099, Mainz</wicri:noRegion><wicri:noRegion>Mainz</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Molecular Biology</title><title level="j" type="abbrev">YJMBI</title><idno type="ISSN">0022-2836</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">298</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="21">21</biblScope><biblScope unit="page" to="34">34</biblScope></imprint><idno type="ISSN">0022-2836</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-2836</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3D reconstruction</term><term>CTF</term><term>FSCF</term><term>FU</term><term>Haliotis tuberculata</term><term>HtH</term><term>KLH</term><term>MSA</term><term>TEM</term><term>cryoelectron microscopy</term><term>hemocyanin</term><term>subunit dimer</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Angular reconstitution</term><term>Biol</term><term>Breemen</term><term>Bruggen</term><term>Central tier</term><term>Characteristic views</term><term>Class averages</term><term>Collar components</term><term>Cryoelectron</term><term>Cryoelectron microscopy</term><term>Decamer</term><term>Didecamer</term><term>Didecamers</term><term>Dimer</term><term>Electron density</term><term>Electron microscopy</term><term>Fourier shell correlation function</term><term>Fscf</term><term>Functional units</term><term>Gastropod</term><term>Gebauer</term><term>Haliotis</term><term>Haliotis tuberculata</term><term>Helix pomatia</term><term>Hemocyanin</term><term>Hemocyanin molecule</term><term>Hemocyanins</term><term>Holde</term><term>Holde miller</term><term>Horizontal sections</term><term>Hth1</term><term>Hth1 decamer</term><term>Hth1 didecamer</term><term>Hth1 subunit dimer</term><term>Hth2</term><term>Image processing</term><term>Interactive extraction</term><term>Keller</term><term>Keyhole</term><term>Keyhole limpet hemocyanin</term><term>Klh1</term><term>Klh2</term><term>Klug</term><term>Lamy</term><term>Lieb</term><term>Limpet</term><term>Markl</term><term>Mellema</term><term>Mellema klug</term><term>Micrographs</term><term>Micron</term><term>Molecule</term><term>Molluscan</term><term>Molluscan hemocyanin decamer</term><term>Molluscan hemocyanins</term><term>Morphological unit</term><term>Morphological units</term><term>Oblique</term><term>Octopus</term><term>Octopus hemocyanin</term><term>Orlova</term><term>Outer surface</term><term>Pomatia</term><term>Power spectrum</term><term>Projection image</term><term>Quaternary</term><term>Quaternary structure</term><term>Sohngen</term><term>Stereo pairs</term><term>Structural feature</term><term>Structural features</term><term>Subunit</term><term>Subunit dimer</term><term>Thin layer</term><term>Third tier</term><term>Threshold curve</term><term>Tier</term><term>Tuberculata</term><term>Unstained molecules</term><term>Upper decamer</term><term>White arrows</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A 12 Å resolution three-dimensional density map of the Haliotis tuberculata hemocyanin type 1 (HtH1) didecamer has been obtained by cryoelectron microscopy of unstained molecules and angular reconstitution. The dyad symmetry of the 8 MDa D5 HtH1 didecamer, formed by the pairing of two asymmetric 4 MDa ring-like C5 decamers, is emphasised. The major and minor surface helical grooves of the didecamer are well defined, in agreement with earlier data on molluscan hemocyanins. The location of the obliquely orientated repeating unit, a subunit dimer, within the decamer has been defined. Following interactive extraction of this dimer, several new structural features of the dimer and of the subunit have now emerged with improved detail. The subunit dimer possesses pseudo 2-fold symmetry, resulting from the steric arrangement of the wall domains/functional units (FUs-abcdef) of the two subunits. The arc and collar FUs (g and h) depart from this inherent 2-fold symmetry and are thereby responsible for the asymmetry of the C5 decamer, with the internalised collar/arc complex at one edge of the decamer. The FU heterodimers forming the wall morphological units have a hollow centre, and thus create a series of repeating channels that extend within the wall through all three tiers of the decamer. The connections between the wall and the arc are defined with improved clarity, and evidence is provided to indicate that the arc and collar FU pairs have a homodimeric composition (gg and hh, respectively). Two possibilities for the subunit path within the subunit dimer are presented, which correlate with the available structural, immunolabelling and protease cleavage data from HtH1 and other molluscan hemocyanins.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Meissner, Ulrich" sort="Meissner, Ulrich" uniqKey="Meissner U" first="Ulrich" last="Meissner">Ulrich Meissner</name></noRegion><name sortKey="Dube, Prakash" sort="Dube, Prakash" uniqKey="Dube P" first="Prakash" last="Dube">Prakash Dube</name><name sortKey="Harris, J Robin" sort="Harris, J Robin" uniqKey="Harris J" first="J. Robin" last="Harris">J. Robin Harris</name><name sortKey="Markl, Jurgen" sort="Markl, Jurgen" uniqKey="Markl J" first="Jürgen" last="Markl">Jürgen Markl</name><name sortKey="Stark, Holger" sort="Stark, Holger" uniqKey="Stark H" first="Holger" last="Stark">Holger Stark</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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